לאתר שלנו בעברית, אנא הקישו כאן
Lab Members :
Dr. Mohamad Abu-Abied Ph.D. - Research Engineer
Ori Serero MSc Student
Avi Eliyahu MSc Student
Sapir Hagay BSc Student
Sarah Sharf, volunteer
Former lab members:
Maoz Lahav, MSc student , finished 2004
Shelly Zaffriar, MSc student, finished 2005
Baruch Zimerman, Post Doc, finished 2006
Lior Golomb, MSc student, finished 2007
Michal Halpert, MSc student finished 2008
Dr. Dror Avisar, Post Doc, finished 2010
David Szwerdszarf, finished 2010
Dr. Yoni Maskovitch, Post Doc, finished 2011
Osnat Altshuler, Msc student, finished 2013
Oxana Rogovoy, Post Doc, finished 2012
Aviv Levy, MSc student, finished 2013
Raj Kumar Thapa, MSc student, finished 2013
Inna Mordehaev, Bsc student, finished 2013
Marina Grumberg MSc finished 2014
Saar Leninkron BSc finished 2014
The role of the cytoskeleton in adventitious root organogenesis
MTs are assembled into the spindle apparatus and the phragmoplast to allow plant cells to execute mitosis/meiosis and cytokinesis. They are organized into distinct cortical arrays in interphase cells, in which parallel MTs regulate the orientation of cellulose microfibril deposited in nascent cell wall, which consequently dictates cell shapes. Although MTs play essential roles in governing cell division and cell elongation, some evidence suggests MTs bear additional functions in programs related to organogenesis. By forming landmarks for the cellulose synthase complex microtubules affect the properties of cell walls, and in turn they can respond to external forces that depend on the properties of cell walls. The feedback loop between MTs and cell wall mechanical properties appears to be linked to auxin signaling. Auxin, induces cell wall acidification and cell wall loosening. In turn, wall mechanical properties have been shown to affect the localization of the auxin transporter PIN1 in the plasma membrane and therefore auxin transport and accumulation. Organogenesis of new primordia depends on auxin accumulation but it can also be triggered by local cell wall loosening. Taken together it suggests a feedback loop between microtubules, cell walls and auxin signaling that functions during plant organogenesis. We study the role of microtubules in adventitious root organogenesis beyond their classical roles in cell division and elongation. (continue to photo gallery)
The function of myosins in plant cell biology
Plant myosins are implicated in various cellular activities, such as cytoplasmic streaming, plasmodesmata function, organelle motility and positioning, ER dynamic rearrangements, cytokinesis, endocytosis, and viral cell to cell spreading. Acto-myosin mediated cytoplasmic streaming, found in certain algal cells, can reach velocities of up to100 µm/sec, which is the fastest known myosin-mediated movement in any eukaryotic system. The Arabidopsis myosin family comprises 17 members: 13 myosin XI variants and four myosin VIII variants. We study specificities and redundancies in plant myosin function. We follow the role of specific myosins in organelles movement and in cytoplasmic streaming (continue to photo gallery)
The effect of natural compounds on the cytoskeleton
The cytoskeleton is a network of filamentous protein polymers that regulate various aspects of the lives of eukaryotic cells, among them shape, adhesion, motility and division. Plant-derived compounds have made a major contribution to human medicine for thousands of years and continue to serve as an indispensable source of therapeutic substances. Drug discovery from medicinal plants has yielded compounds that act on the cytoskeleton; some of these are in clinical use, including colchicines, the vinca alkaloids, taxanes, and podophyllotoxins. Compounds that are produced in living organisms usually have a complex chemical structure that is naturally designed to interact with cellular components. Therefore, although high-throughput screens based on mixtures of natural products are more difficult to handle and less favored by the pharmaceutical companies, they still have recognized, highly valuable advantages. We have found several plants derived natural compounds affecting the cytoskeleton. (continue to photo gallery)
The loss of rooting capability during the juvenile to mature phase change in woody plants
Rooting capacity is one of the economically important traits lost during the juvenile to mature phase change in woody plants. The difficulties in the propagation of promising clones of woody plants such as rootstocks of fruit trees, ornamental woody plants, and forest trees, hamper breeding programs that depend on the production of rooted cuttings. Four phases of plant maturation have been defined: (1) the embryonic phase, (2) the post-embryonic juvenile vegetative phase, (3) the mature vegetative phase, and (4) the mature reproductive phase. In woody plants, the juvenile traits, including rooting capability, are expressed at the bottom of the stem throughout the life of the plant and are sharply or gradually being replaced by maturity traits, including rooting incapability, towards the upper part of the main stem and the branches. Adventitious root formation is a complex process, in which roots differentiate and regenerate from non-root tissues. Adventitious root formation in cuttings is often described to occur in four steps: (1) cell de-differentiation, (2) cell division, (3) development of root primordia, and (4) root emergence, in each of which auxin plays a major role. Histological analysis of woody plants induced to form adventitious roots revealed that whereas in both juvenile and mature tissues cell division was induced (step 2), differentiation of root primordium (step 3) occurred efficiently in juvenile cutting but much less, if at all, in mature cuttings. Therefore it seems that the shift from cell division to cell differentiation is hampered in mature cuttings.We study the molecular basis of the loss of rooting capability in cuttings from mature woody plants. (continue to photo Gallery).
List of publications:
1. Sadot E, Marx R, Barg J, Beher L, Ginzburg I (1994) Complete Sequence of 3'-untranslated region of Tau from rat central nervous system. Implications for mRNA heterogeneity. J. Mol. Biol. 241(2):325-331.
2. Sadot E, Barg J, Rasouly D, Lazarovici P, Ginzburg I (1995) Short- and long-term mechanisms of tau regulation in PC12 cells.J. Cell Sci. 108:2857-2864.
3. Behar L, Marx R, Sadot E, Barg J, Ginzburg I (1995) Cis-acting signals and trans-activating proteins are involved in tau nRNA targeting into neurites of differentiating neuronal cells. Int. J. Dev. Neurosci. 13 (2):113-127.
4. Fisher A, Heldman E, Gurwitz D, Haring R, Karton Y, Meshulam H, Pittel Z, Marciano D, Brandeis R Sadot E, Barg J, Pinkas-Kramarski R, Vogel Z, Ginzburg I, Treves TA, Verchovsky R, Klimowsky S, Korezyn AD (1996) M1 agonists for the treatment of Alzheimer's disease. Novel properties and clinical update. Ann N Y Acad. Sci. 777:189-196.
5. Sadot E, Gurwitz D, Barg J, Behar L, Ginzburg I, Fisher A (1996) Activation of m1 muscarinic acetylcholine receptor regulates tau phosphorylation in transfected PC12 cells. J. Neurochem. 66(2):877-880.
6. Sadot E, Heicklen-Klein A, Barg J, Lazarovici P, Ginzburg I (1996) Identification of a tau promoter region mediating tissue-specific-regulated expression in PC12 cells. J. Mol. Biol. 256(5):805-812.
7. Sadot E, Jaaro H, Seger R, Ginzburg I (1998) Ras-signaling pathways: positive and negative regulation of tau expression in PC12 cells. J. Neurochem. 70(1):428-431.
8. Simcha I, Shtutman M, Salomon D, Zhurinsky J, Sadot E, Geiger B, Ben-Ze'ev A (1998) Differential nuclear translocation and transactivation potential of ?-catenin and plakoglobin. J. Cell Biol. 141(6):1433-1448.
9. Levenberg S, Sadot E, Goichberg P, Geiger B (1998) Cadherin-mediated transmembrane interactions. Cell Adhes. Commun. 6(2-3):161-170.
10. Sadot E, Simcha I, Shtutman M, Ben-Ze'ev A, Geiger B (1998) Inhibition of ?-catenin-mediated transactivation by cadherin derivatives. Proc. Natl. Acad. Sci. USA 95(26);15339-15344.
11. Sadot E, Simcha I, Iwai K, Ciechanover A, Geiger B and Ben-Ze'ev A (2000) Differential interaction of plakoglobin and ?-catenin with the ubiquitin-proteasome system. Oncogene, 19;1992-2001.
12. Simcha I, Kirkpatrick C, Sadot E, Shtutman M, Polevoy G, Geiger B, Peifer M and Ben-Ze'ev A. (2001) Cadherin sequences that inhibit ?-catenin signaling: a study in yeast and mammalian cells. Mol Biol Cell 12;1177-1188.
13. Sadot E, Geiger B, Oren M, and Ben-Ze'ev A, (2001) Down-regulation of ?-catenin by activated p53. Mol. Cell Biol. 21(20); 6768-6781.
14. Rozenblatt-Rosen O, Mosonego-Ornan E, Sadot E, Madar-Shapiro L, Sheinin Y, Ginsberg D, Yayon A. (2002) Induction of chondrocyte growth arrest by FGF: transcriptional and cytoskeletal alterations.J. Cell Sci. 115;553-562.
15. Sadot E, Conacci-Sorrell M, Zhurinsky J, Shnizer D, Lando Z, Zharhary D, Kam Z, Ben-Ze'ev A, and Geiger B. (2002) Regulation of S33,S37 phosphorylated ?-catenin in normal and transformed cells. J. Cell Sci. 115;2271-2780.
16. Lahav M, Abu-Abied M, Belausov E, Schwartz A and Sadot E. (2004) Microtubules of guard cells are light sensitive. Plant Cell Physiol. 45(5) 573-582.
17. Bloch D, Lavy M, Efrat Y, Efroni I, Bracha-Drori K, Abu-Abied M, Sadot E. and Yalovsky S. (2005) Ectopic Expression of an Activated RAC in Arabidopsis Disrupts Membrane Cycling. Mol Biol Cell. 2005 Apr;16(4):1913-27.
18. Zaffryar S, Zimerman B, Abu-Abied M, Belausov E, Lurya G, Vainstein A, Kamenetsky R, and Sadot E. (2007) Developmental specific association of amyloplasts with microtubules in scale cells of Narcissus tazetta. rotoplasma. 230(3-4):153-63.
19. Yasuor, H., Abu-Abied, M., Belausov, E., Madmoni, A., Sadot, E., Riov, J. and Rubin, B. (2006) Glyphosate-induced antherindehiscence in cotton is partially temperature-dependent and involves cytoskeleton and secondary wall modifications and auxin accumulation. Plant Physiol. 141(4):1306-15.
20. Abu-Abied, M., Golomb, L., Belausov, E., Huang, S., Geiger, B., Kam, Z., Staiger,C.J. and Sadot E. (2006) Identification of Plant Cytoskeleton-Interacting Proteins by Screening for Actin Stress Fiber Association in Mammalian Fibroblasts. Plant J. 48;367-379.
21. Golomb L, Abu-Abied M, Belausov E, Sadot E. (2008) Different subcellular localizations and functions of Arabidopsis myosin VIII.BMC Plant Biol. 2008 Jan 8;8:3.
22. Abu-Abied M, Avisar D, Belausov E, Holdengreber V, Kam Z, Sadot E. (2009) Identification of an Arabidopsis unknown small membrane protein targeted to mitochondria, chloroplasts, and peroxisomes. Protoplasma 236(1-4) 3-12.
23. Avisar, D., Abu-Abied, M., Belausov, E., Sadot, E., Hawes C. and Sparkes I.A. (2009) A comparative study on the involvement of 17 Arabidopsis myosin family members on the motility of Golgi and other organelles. Plant Physiol. 150(2) 700-709.
24. Chaimovitsh D, Abu-Abied M, Belausov E, Rubin B, Dudai N, Sadot E.(2010) Microtubules are an intracellular target of the plant terpene citral. Plant J. 61(3):399-408.
25. Halpert M?, Abu-Abied M, Avisar D, Moskovitz Y, Altshuler O, Cohen A, Weissberg M, Riov J, Gottlieb H.E, Perl A and Sadot E (2011) Rac-dependent doubling of HeLa cell area and impairment of cell migration and cell cycle by compounds from Iris germanicaProtoplasma. 248(4):785-797.
26. Sorek N, Gutman O, Bar E, Running M.P, Lewinsohn E, Ori N, Sadot E, Henis Y.I and Yalovsky S. (2011) The effects of prenylation and s-acylation on G proteins membrane interaction dynamics and function. Plant Physiol. 155(2):706-20.
27. Avisar D., Abu-Abied M., Belausov E. and Sadot E. (2011) Myosin XIK is a major player in cytoplasm dynamics and is regulated by two amino acids in its tail. J. Exp. Bot. 63(1):241-9.
28. Chaimovitsh D., Rogovoy (Stelmakh) O., Altshuler O., Belausov E., Abu-Abied M., Rubin B., Sadot E. and Dudai N. (2011) The relative effect of citral on mitotic microtubules in wheat roots and BY2 cells. Plant Biol. 14(2):354-364.
29. Abu-Abied, M. Szwerdszarf, D. Mordehaev, I. Levy, A. Rogovoy (Stelmakh), O. Belausov, E. Yaniv, Y. Uliel, S. Katzenellenbogen, M. Riov, J. Ophir, R. and Sadot E. (2012) Microarray analysis revealed upregulation of nitrate reductase in juvenile cuttings of Eucalyptus grandis which correlated with increased nitric oxide production and adventitious root formation. Plant J. 71(5):787-99
30. Riov J., Szwerdszarf D., Abu-Abied M., Sadot E. (2013) The molecular mechanisms involved in adventitious root formation. Plant Roots: The Hidden Half, 5th edition, Eds. A. Eshel and T. Beeckman, Taylor and Francis, LLC.
31. Poraty-Gavra L., Zimmermann P., Haigis S., Bednarek P., Hazak O., Stelmakh OR., Sadot E,. Schulze-Lefert P., Gruissem W., Yalovsky S. (2013) The Arabidopsis Rho of plants GTPase AtROP6 functions in developmental and pathogen response pathways.Plant physiol. 161(3):1172-1188
32. Altshuler O., Abu-Abied M., Chaimovitsh D., Shechter A., Frucht H., Dudai N. and Sadot E. (2013) Enantioselective Effects of (+)- and (-)-Citronellal on Animal and Plant Microtubules. J. Nat. Prod. 76(9):1598-1604
33. Sadot E. (2013) Plant compounds acting on the cytoskeleton. Applied Plant Cell Biology; Cellular Tools and Approaches for Plant Biotechnology. Eds: Peter Nick and Zdenek Opatrný. Springer. Pp. 301-323.
34. Li J, Staiger BH, Henty-Ridilla JL, Abu-Abied M, Sadot E, Blanchoin L, Staiger CJ. (2014) The availability of filament ends modulates actin stochastic dynamics in live plant cells. Mol Biol Cell. 25(8):1263-1275.
35. Levy A., Szwerdszarf D., Abu-Abied M., MordehaevI., Yaniv Y., Riov J., Arazi T., and Sadot E (2014) Profiling microRNAs in Eucalyptus grandis reveals no mutual relationship between alterations in miR156 and miR172 expression and adventitious root induction during development. BMC Genomics 25;15(1):524
36. Abu-Abied M, Szwerdszarf D, Mordehaev I, Yaniv Y, Levinkron S, Rubinstein M, Riov J, Ophir R, Sadot E (2014) Gene expression profiling in juvenile and mature cuttings of Eucalyptus grandisreveals the importance of microtubule remodeling during adventitious root formation. BMC Genomics. 2014 Sep 30;15:826.
37. Buchnik L, Abu-Abied M, Sadot E (2014) Role of plant myosins in motile organelles: is a direct interaction required? J Integr Plant Biol. 2015 Jan;57(1):23-30.
38. Henn A, Sadot E. (2014) The unique enzymatic and mechanistic properties of plant myosins. Curr Opin Plant Biol. 2014 Dec;22:65-70.
39. Abu-Abied, M., Rogovoy (Stelmakh), O., Mordehaev, I., Grumberg, M. Elbaum, R., Wasteneys, G.O. and Sadot, E. (2015) Dissecting the contribution of microtubules behavior in adventitious root induction J Exp Bot. 66(9):2813-24.
40. Abu-Abied M, Mordehaev I, Sunil Kumar GB, Ophir R, Wasteneys GO, Sadot E. (2015) Analysis of Microtubule-Associated-Proteins during IBA-Mediated Adventitious Root Induction Reveals KATANIN Dependent and Independent Alterations of Expression Patterns. PLoS One. 2015 Dec 2;10(12).
41. Chaimovitsh, D., Shechter, A., Abu-Abeid, M., Rubin, B., Sadot, E., and Dudai, N.(2016) Herbicidal Activity of Monoterpenes Is Associated with Disruption of Microtubules Functionality and Membrane Integrity. Weed Sci. 65(1):19-30.