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About Institutes Public Relations Technology Transfer Students\International Information Center (Library) Administration
שלח באימייל הדפס
Name: Michal Oren-Shamir, Prof. (Researcher)
Units: Plant SciencesOrnamental Plants and Agricultural Biotechnology
Research Interests / Job description  
Tel:  ‎‎03-9683840 ‎‎03-9683841
Email:    vhshamir@volcani.agri.gov.il
Research Interests / Job description
Phenylpropanoids in plants as fragrance, color and protection
Research Interests 
Revealing the enzymatic process of active anthocyanin degradation

How are anthocyanins degraded in plants? What role does the pigment catabolism process play in the final determination of pigment concentration in flower foliage or fruit? These are two of the major current research questions of my group. In contrast to the detailed knowledge available on the biosynthesis of anthocyanins, very little is known about the stability and degradation of these pigments in plant tissue.
Our model system is Brunfelsia calycina flowers, which change color from dark purple to white within two days. We are characterizing in planta anthocyanin turnover and degradation. In particular, we have identified, for the first time, an anthocyanin degrading enzyme, responsible for flower color change. We are also characterizing the metabolomics and genomic changes occurring during the change of color in Brunfelsia flowers, accompanied by production of flower fragrant compounds.



Phenylpropanoid pathways affecting pigmentation and fragrance

We expanded our studies to interactions between anthocyanin and other phenyproanoid pathways, especially the benzenoid pathway responsible for production of fragrant compounds. Parallel to the degradation of anthocyanins, Brunfelsia flowers produce fragrant volatiles, making this flower an interesting model for studying the relationship between pigment and fragrance production pathways. We use a number of profiling approaches to examine anthocyanin composition, volatile compound production, metabolite accumulation, and transcriptional and protein changes occurring during and after flower opening. 
            A second approach for studying the link between the different phenylpropanoid pathways (including anthocyanin pigments and benzenoid folatiles) is overexpressing the production of aromatic amino acids and in particular phenylalanine. The tool that we use for overproducing phenylalanine, the source for production of both anthocyanins and benzenoids is a feedback insensitive enzyme along the shikimate pathway. We are currently studying the metabolic and genomic effect of increased phenylalanine concentrations in genetically engineered petunia, lisianthus and in a grape cell culture. 




Applied research for improving pigmentation in ornamentals 

In order to improve and increase the quality of horticultural products, we develop solutions for optimization of anthocyanin pigmentation, based on characterization of the best conditions for anthocyanin accumulation in ornamental crops. This research includes testing best quality greenhouse covers for maximum pigmentation, and treatments for increasing pigmentation in plants, including hormonal treatments for inducing biosynthesis and treatments for stabilizing the anthocyanins in the plant tissues.

Effect of colored nets on the yield and quality of ornamental crops

The technology of plant production under colored shade nets, instead of the black neutral net,  is based on fact that plants are extremely sensitive to light quality, and that the adaptation to light changes occurs via a wide and complex network of photoreceptors. We were the first to use photoselective nets to improve the quality and yield of crops and are continuing experiments using this technology. We showed that by changing the direction and quality of the light by filtering sunlight through photoselective nets, we can improve the quality of ornamentals and increase their yield. At present we are focusing our work on testing the effect of shading mother-plants with photoselective nets, on the rooting of their cuttings.




Current group members

Rinat Ovadia, M.Sc. Research Assistant

Dr. Stalin Nadarajan, Postdoc.

Dr. Anita Kumari, Postdoc.

Dr. Dayakar Boddupally, Postdoc.

Tzlil Shaharabani, undergraduate student


Former group members

Ada Nissim-Levi, Research Assistant

Dr. Varun Gupta, Postdoc.

Dr. Fang Fang, Postdoc.

Dr. Moran Oliva, Postdoc.

Dr. Sangram Keshari Lenka, Postdoc.

Dr. Gadi Zipor, Postdoc.

Dr. Bhaskaran Sinilal, Postdoc.

Ru Wang, Ph.D student

Ayelet Bar-Akiva, Ph.D. student

Erel Hatan, M.Sc student

Sonia Ehi-Eromosele, M.Sc student

Yuval Elazari, MSc. Student

Raya Liberman, MSc. Student

Hila Vaknin, M.Sc. student

Liat Shahar, M.Sc. student

Maya Sapir, M.Sc. student

Zohar Frieman, MSc. Student

Gali Shreiber, M.Sc. student

Gal Dela, M.Sc student

Liat Shaked-Sachray, M.Sc student

Eddy Wiseman, undergraduate student

Lilach Farkash, undergraduate student

David Hamburger, undergraduate student

Barak Dror, undergraduate student

Shimrit Zinzinatos, undergraduate student

Netta Bashari, undergraduate student

Shir Elbaz, undergraduate student



Selected publications

Oren-Shamir M. and Levi-Nissim A. (1997). Temperature effect on the leaf pigmentation of Cotinus coggygria 'Royal Purple'. J. of Hort. Sci. 72 (3): 425-432.

Oren-Shamir M. and Levi-Nissim A. (1997). UV-Light effect on the leaf pigmentation of Cotinus coggygria 'Royal Purple'. Sci. Hort. 71: 59-66.

Oren-Shamir M., Shaked-Sachray L, Nissim-Levi A and Ecker R. (1999). Anthocyanin pigmentation of lisianthus flower petals. Plant Sci. 140: 81-86.

Oren-Shamir M. and Nissim-Levi A. (1999). Temperature and gibberellin effect on growth and anthocyanin pigmentation in Photinia leaves. J. Hortic. Sci. Biotechnol. 74: 355-360.

Oren-Shamir M., Weiss D., and Shaked-Sachray L., Nissim-Levi A. (2000). Effect of growth temperature on Aster flower development HortScience. 35: 28-29.

Oren-Shamir M., Dela G., Ovadia R., Nissim-Levi A., Philosoph-Hadas S. and Meir M. (2001). Differentiation between petal blueing and senescence of cut 'Mercedes' rose flowers. J. Hortic. Sci. Biotechnol. 76: 195-200.

Oren-Shamir M., Gussakovsky E. E., Shpiegel E., Nissim-Levi A., Ratner K., Ovadia R., Giller Y. and Shahak Y. (2001). Coloured shade nets can improve the yield and quality of green decorative branches of Pittosporum variegatum. J. Hortic. Sci. Biotechnol. 76: 353-361.

Shaked-Sachray L.S, Weiss D., Reuveni M., Nissim-Levi A. and Oren-Shamir M. (2002). Increased anthocyanin accumulation in aster flowers at elevated temperatures due to Magnesium treatment. Physiol. Plant. 114: 559-565.

Dela G., Or E., Ovadia R., Nissim-Levi A., Weiss D. and Oren-Shamir M. (2003). Changes in anthocyanin concentration and composition in ‘Jaguar' rose flowers due to transient high-temperature conditions. Plant Sci. 164: 333-340.

Nissim-Levi A.S, Kagan S., Ovadia R. and Oren-Shamir M. (2003). Effects of temperature, UV-light and magnesium on anthocyanin pigmentation in Cocoplum leaves. J. Hortic. Sci. Biotechnol. 78: 61-64.

Borovsky Y., Oren-Shamir M., Ovadia R., De Jong W. and Paran I. (2004). The A locus that controls anthocyanin accumulation in pepper encodes a MYB transcription factor homologous to Anthocyanin2 of Petunia Theor. Appl. Genet. 109: 23-29.

Ben-Yehudah G., Korchinsky R., Redel G., Ovadia R, Oren-Shamir M., and Cohen Y.(2005). Colour accumulation patterns and anthocyanin biosynthetic pathway in 'Red Delicious' apple variants. J. Hortic. Sci. Biotechnol. 80: 187-192.

Vaknin H.S, Bar-Akiva A., Ovadia R., Nissim-Levi A., Forer I., Weiss D. and Oren-Shamir M. (2005). Active anthocyanin degradation in Brunfelsia calycina (Yesterday-Toda-Tomorrow) flowers. Planta 222: 19-26.

Nissim-Levi A.S, Ovadia R., Forer I. and Oren-Shamir M. (2007). Increased anthocyanin accumulation in ornamental plants due to magnesium treatment. J. Hortic. Sci. Biotechnol. 82: 481-487.

Nissim-Levi A.S, Farkash L., Hamburger D., Ovadia R., Forer I., Kagan S. and Oren-Shamir M. (2008). Light-scattering shade net increases branching and flowering in ornamental pot plants. J. Hortic. Sci. Biotechnol. 83: 9-14.

Sapir M., Oren-Shamir M., Ovadia R., Reuveni M., Evenor D., Tadmor Y., Nahon S., Shlomo H., Chen L., Meir A. and Levin I. (2008). Molecular aspects of anthocyanin fruit tomato in relation to high pigment-1. J. Hered. 99(3): 292-303.‏

Ovadia R., Dori I., Nissim-Levi A., Shahak Y. and Oren-Shamir M. (2009). Coloured shade-nets influence stem length, time to flower, flower number and inflorescence diameter in four ornamental cut-flower crops. J. Hortic. Sci. Biotechnol. 84: 161-166.

Lavi N., Tadmor Y., Meir A., Bechar A., Oren-Shamir M. Ovadia R., Reuveni M., Nahon S., Shlomo H., Chen, L. and Levin I. (2009). Characterization of the Intense Pigment tomato genotype emphasizing targeted fruit metabolites and chloroplast biogenesis. J. Agri. Food Chem. 57: 4818-4826

Lurie S., Oren-Shamir M., Ovadia R., Nissim-Levi A., Kaplunov T., Zutahy Y., Weksler H., and Lichter A. (2009). Abscisic acid improves color development of ‘Crimson Seedless' grapes both in the vineyard and on detached berries. J. Hortic. Sci. Biotechnol. 84: 639-644.

Segev A., Badani H., Kapulnik Y., Shomer I., Oren-Shamir M. and Galili S. (2010). Determination of polyphenols, flavonoids and antioxidant capacity in colored chickpea (Cicer arietinum L.) J. Food Sci. 75: S115-119.

Liberman R., Shahar L., Nissim-Levi A., Evenor D., Reuveni M. and Oren-Shamir M. (2010). Shoot regeneration from leaf explants of Brunfelsia calycina. Plant Cell Tissue Organ Cult. 100: 345-348.

Bar-Akiva A., Ovadia R., Rogachev I, Bar-Or C., Bar E., Freiman Z., Nissim-Levi A., Gollop N., Lewinshon E., Aharoni A., Weiss D., Koltai H and Oren-Shamir M. (2010). Metabolic networking in Brunfelsia calycina petals after flower opening. J. Exp. Bot. 61: 1393-1403.

Sinilal B., Ovadia R., Nissim-Levi A., Perl A., Weissberg M. and Oren-Shamir M. (2011). Increased accumulation and decreased catabolism of anthocyanins in red grape cell suspension culture following magnesium treatment. Planta 234: 61-71.

Schreiber G., Reuveni M., Evenor D., Oren-Shamir M., Ovadia R., Sapir-Mir M, Bootbool-Man A, Nahon S, Shlomo H., Chen L, and Levin I (2011). Anthocyanin1 from Solanum chilense is more efficient in accumulating anthocyanin metabolites than its Solanum lycopersicum counterpart in association with the Anthocyaninin fruit phenotype of tomato. Theor. Appl. Genet. 124: 295-307.

Ovadia R., Oren-Shamir M., Kapltinov T., Zutahy Y., Lichter A. and Lurie S. (2013). Effects of plant growth regulators and high temperature on colour development in ‘Crimson Seedless' grapes. J. Hort. Sci. and Biotech. 88, 387-392.

Nissim-Levi A.S, Kagan S, Ovadia R., Forrer I., Riov J. and Oren-Shamir M. (2013). Effects of temperature and auxin treatment on fruit set and pigmentation of Dodoneae 'Dana'. Sci. Hort. 160, 172-176.

Nissim-Levi A.S, Ovadia R., Kagan S. and Oren-Shamir M. (2014). Shading stock plants with photoselective nets affects the yield and rooting quality of their cuttings. J. Hortic. Sci. Biotechnol. 89: 693-699.

Zipor G.S, Duarte P, Carqueijeiro I., Shahar L., Ovadia R., Teper-Bamnolker P., Eshel D., Levi Y., Doron-Faigenboim A., Sottomayor M. and Oren-Shamir M. (2014). In planta anthocyanin degradation by a vacuolar class III peroxidase in Brunfelsia Calycina flowers. New Phytol. 205(2): 653-665.

Oliva, M., Ovadia, R., Perl, A., Bar, E., Lewinsohn, E., Galili, G., and Oren?Shamir, M. (2015). Enhanced formation of aromatic amino acids increases fragrance without affecting flower longevity or pigmentation in Petunia×hybrida. Plant Biotechnol. J. 13(1): 125-136.‏

Fang F., Zhang X.L., Luo H.H., Zhou J.J., Gong Y.H., Li W.J., Shi Z.W., He, Q., Wu Q., Li L., Jiang L.L., Cai Z.G., Oren-Shamir M., Zhang Z.Q., and Pang X.Q. (2015). An intracellular laccase is responsible for epicatechin-mediated anthocyanin degradation in litchi fruit pericarp. Plant Physiol. 169(4): 2391-2408.

Peled-Zehavi H., Oliva M., Xie Q., Tzin V., Oren-Shamir M., Aharoni A. and Galili G. (2015). Metabolic engineering of the phenylpropanoid and its primary, precursor pathway to enhance the flavor of fruits and the aroma of flowers. Bioengineering 2(4): 204-212.

Manela N., Oliva M., Ovadia R., Sikron-Persi N., Ayenew B., Fait A., Galili G., Perl A., Weiss D. and Oren-Shamir M. (2015). Phenylalanine and tyrosine levels are rate-limiting factors in production of health promoting metabolites in Vitis vinifera cv. Gamay Red cell suspension. Front. Plant Sci. 6: 538.‏

Oliva M., Bar E., Ovadia R., Perl A., Galili G., Lewinsohn E., and Oren-Shamir, M. (2017). Phenylpyruvate contributes to the synthesis of fragrant benzenoids-phenylpropanoids in Petunia×hybrida flowers. Front. Plant Sci. 8: 769.

Fang F., Oliva M., Ehi?Eromosele S., Zaccai M., Arazi T. and Oren-Shamir, M. (2018). Successful floral dipping transformation of post anthesis lisianthus (Eustoma grandiflorum) flowers. Plant J. 96(4): 869-879.‏‏

Fogelman E., Oren-Shamir M., Hirschberg J., Mandolino G., Parisi B., Ovadia R., Tanami Z., Faigenboim A. and Ginzberg, I. (2019). Nutritional value of potato (Solanum tuberosum) in hot climates: anthocyanins, carotenoids, and steroidal glycoalkaloids. Planta 1-13.‏

Sudheeran P. K., Love C., Feygenberg O., Maurer D., Ovadia R., Oren-Shamir M. and Alkan N. (2019). Induction of red skin and improvement of fruit quality in ‘Kent','Shelly' and ‘Maya' mangoes by preharvest spraying of prohydrojasmon at the orchard. Postharvest Biol. Technol. 149: 18-26.‏

Nissim-Levi A., Kitron M., Nishri Y., Ovadia R., Forer I., and Oren-Shamir M. (2019). Effects of blue and red LED lights on growth and flowering of Chrysanthemum morifolium. Sci. Hort. 254: 77-83.‏

Khunmuang S., Kanalyanarat S., Wong-Agree C., Meir, S. Philosoph-Hadas S., Oren-Shamir M., Ovadia, R. and Buanong M. (2019). Ethylene induces a rapid degradation of petal anthocyanins in cut Vanda ‘Sansai Blue' orchid flowers. Front. Plant Sci. 10: 1004.

Oliva M., Hatan E., Kumar V., Galsurker O., Nissim-Levi A., Ovadia, R., Galili G. Lewinsohn E., Elad Y., Alkan N. and Oren-Shamir M. (2020). Increased phenylalanine levels in plant leaves reduces susceptibility to Botrytis cinerea. Plant Sci. 290: 110289.

Sudheeran P.K., Ovadia R., Galsarker O., Maoz I., Sela N., Maurer D., Fegenberg O., Oren-Shamir M. and Alkan N. (2020). Glycosylated flavonoids: fruit's concealed antifungal arsenal. New Phytiol. 225: 1788-1798.

Patel M.K., Maurer D., Feygenberg O., Ovadia A., Elad Y., Oren-Shamir M. and Alkan N. (2020). Phenylalanine: A promising inducer of fruit resistance to postharvest pathogens. Foods 9(5): 646.

Kumar V., Hatan E., Bar E., Davidovich-Rianati R., Doron-Faigenboim A., Spitzer-Rimon B., Elad Y., Alkan N., Lewinsohn E. and Oren-Shamir M. (2020). Phenylalanine increases chrysanthemum flower immunity agains Botrytic cinerea attack. Plant J. 104: 226-240.

Fang F., Oliva M., Ovadia R., Nissim-Levi A., Kumar V., Want R., Neeman A., Zaccai M., Lewinsohn E. and Oren-Shamir M. (2021). Increased substrate availability reveals the potential of scentless lisianthus flowers in producing fragrant benzenoid-phenylpropanoids. Physiol. Plant. 172: 19-28.

Sudheeran P.K., Sela N., Carmeli-Weissberg M., Ovadia R., Pnada S., Feygenberg O., Maurer D., Oren-Shamir M., Aharoni A. and Alkan N. (2021). Induced defense response in red mango fruit against Colletotrichum gloeosporioides. Hortic. Res. 8(1): 1-11.

Wang R., Lenka S., Kumar V., Gashu K., Sikron-Persi N., Dynkin I., Weiss D., Perl A., Fait A. and Oren-Shamir M. (2021). Metabolic engineering strategy enables a hundred-fold increase in viniferin levels in Vitis vinifera cv. Gamay Red cell culture. J. Agric. Food Chem. 69(10): 3124-3133.

Wang R., Lenka S., Kumar V., Sikron-Persi N., Dynkin I., Weiss D., Perl A., Fait A. and Oren-Shamir M. (2021). A synchronized increase of stilbenes and flavonoids in metabolically engineered Vitis vinifera cv. Gamay Red cell culture. J. Agric. Food Chem., 69(28): 7922-7931.

Kumar V., Elazari Y., Ovadia R., Bar E., Nissim-Levi, A., Carmi N., Lewinsohn E. and Oren-Shamir M. (2021). Phenylalanine treatment generates scent in flowers by increased production of phenylpropanoid-benzanoid volatiles. Postharvest Biol. Technol., 181: 111657.

Wang R., Kumar V., Sikron-Persi N., Dynkin I., Weiss D., Perl A., Fait A. and Oren-Shamir, M. (2022). Over 1000-fold synergistic boost in viniferin levels by elicitation of Vitis vinifera cv. Gamay Red cell cultures over accumulating phenylalanine. J. Agric. Food Chem., 70(16): 5049-5056.‏

Fanyuk M., Kumar Patel M., Ovadia R., Maurer D., Feygenberg O., Oren-Shamir M. and Alkan N. (2022). Preharvest application of phenylalanine induces red color in mango and apple fruit's skin. Antioxidants, 11(3): 491.‏

Patel M. K., Fanyuk M., Feyngenberg O., Maurer D., Sela N., Ovadia R., Oren-Shamir M. and Alkan N. (2023). Phenylalanine induces mango fruit resistance against chilling injuries during storage at suboptimal temperature. Food Chem., 405: 134909.‏



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