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About Institutes Public Relations Technology Transfer Students\International Library Administration
שלח באימייל הדפס
Name: Michal Oren-Shamir, Ph.D. (Researcher)
Units: Plant SciencesOrnamental Plants and Agricultural Biotechnology
Research Interests / Job description  
Tel:  ‎‎03-9683840 ‎‎03-9683841
Email:    vhshamir@volcani.agri.gov.il
Research Interests / Job description
(1) Revealing the enzymatic process of active anthocyanin degradation.
(2) Phenylpropanoid pathways affecting pigmentation and fragrance
(3) Applied research for improving pigmentation in ornamentals
(4) Effect of colored nets on the yield and quality of ornamental crops
Research Interests 
Revealing the enzymatic process of active anthocyanin degradation

How are anthocyanins degraded in plants? What role does the pigment catabolism process play in the final determination of pigment concentration in flower foliage or fruit? These are two of the major current research questions of my group. In contrast to the detailed knowledge available on the biosynthesis of anthocyanins, very little is known about the stability and degradation of these pigments in plant tissue. 
            Our model system is Brunfelsia calycina flowers, which change color from dark purple to white within two days. We are characterizing in planta anthocyanin turnover and degradation. In particular, we have identified, for the first time, an anthocyanin degrading enzyme, responsible for flower color change. We are also characterizing the metabolomics and genomic changes occurring during the change of color in Brunfelsia flowers, accompanied by production of flower fragrant compounds.




Phenylpropanoid pathways affecting pigmentation and fragrance

We expanded our studies to interactions between anthocyanin and other phenyproanoid pathways, especially the benzenoid pathway responsible for production of fragrant compounds. Parallel to the degradation of anthocyanins, Brunfelsia flowers produce fragrant volatiles, making this flower an interesting model for studying the relationship between pigment and fragrance production pathways. We use a number of profiling approaches to examine anthocyanin composition, volatile compound production, metabolite accumulation, and transcriptional and protein changes occurring during and after flower opening. 
            A second approach for studying the link between the different phenylpropanoid pathways (including anthocyanin pigments and benzenoid folatiles) is overexpressing the production of aromatic amino acids and in particular phenylalanine. The tool that we use for overproducing phenylalanine, the source for production of both anthocyanins and benzenoids is a feedback insensitive enzyme along the shikimate pathway. We are currently studying the metabolic and genomic effect of increased phenylalanine concentrations in genetically engineered petunia, lisianthus and in a grape cell culture. 




Applied research for improving pigmentation in ornamentals 

 In order to improve and increase the quality of horticultural products, we develop solutions for optimization of anthocyanin pigmentation, based on characterization of the best conditions for anthocyanin accumulation in ornamental crops. This research includes testing best quality greenhouse covers for maximum pigmentation, and treatments for increasing pigmentation in plants, including hormonal treatments for inducing biosynthesis and treatments for stabilizing the anthocyanins in the plant tissues.

Effect of colored nets on the yield and quality of ornamental crops

The technology of plant production under colored shade nets, instead of the black neutral net,  is based on fact that plants are extremely sensitive to light quality, and that the adaptation to light changes occurs via a wide and complex network of photoreceptors. We were the first to use photoselective nets to improve the quality and yield of crops and are continuing experiments using this technology. We showed that by changing the direction and quality of the light by filtering sunlight through photoselective nets, we can improve the quality of ornamentals and increase their yield. At present we are focusing our work on testing the effect of shading mother-plants with photoselective nets, on the rooting of their cuttings.




Current group members



Ada Nissim-Levi, Research Assistant
Rinat Ovadia, M.Sc. Research Assistant
Dr. Moran Oliva, Postdoc
Neta Manela, M.Sc student
Barak Dror, undergraduate student
Shimrit Zinzinatos
Niv Grinbaum

Former group members

Liat Shaked-Sachray, MSc student.
Gal Dela, MSc. student.
Hila Vaknin, M.Sc. student
Liat Shahar, M.Sc. student
Maya Sapir, M.Sc. student
Zohar Frieman, MSc. student
Ayelet Bar-Akiva, Ph.D. student
Lilach Farkash, undergraduate student
David Hamburger, undergraduate student
Gali Shreiber, M.Sc. student
Raya Liberman, MSc. student
Dr.B. Sinilal, Postdoc.
Dr. Gadi Zipor, Postdoc.
Eddy Wiseman, undergraduate student

Selected publications

Oren-Shamir M. and Levi-Nissim A. (1997). Temperature effect on the leaf pigmentation of Cotinus coggygria 'Royal Purple'. J. of Hort. Sci. 72 (3), 425-432.

Oren-Shamir M. and Levi-Nissim A. (1997). UV-Light effect on the leaf pigmentation of Cotinus coggygria 'Royal Purple'. Sci. Hort. 71, 59-66.

Oren-Shamir M., Shaked-Sachray L, Nissim-Levi A and Ecker R. (1999). Anthocyanin pigmentation of lisianthus flower petals. Plant Sci., 140, 81-86.

Oren-Shamir M. and Nissim-Levi A. (1999). Temperature and gibberellin effect on growth and anthocyanin pigmentation in Photinia leaves. J. of Hortic. Sci. and Biotech., 74, 355-360.

Oren-Shamir M., Weiss D., and Shaked-Sachray L., Nissim-Levi A. (2000). Effect of growth temperature on Aster flower development HortScience., 35, 28-29

Oren-Shamir M., Dela G., Ovadia R., Nissim-Levi A., Philosoph-Hadas S. and Meir M. (2001).
Differentiation between petal blueing and senescence of cut 'Mercedes' rose flowers. J.of Hort. Sci. and Biotech., 76, 195-200.

Oren-Shamir M., Gussakovsky E. E., Shpiegel E., Nissim-Levi A., Ratner K., Ovadia R., Giller Y. and Shahak Y. (2001). Coloured shade nets can improve the yield and quality of green decorative branches of Pittosporum variegatum. J. of Hort. Sci. and Biotech., 76: 353-361.

Shaked-Sachray L.S, Weiss D., Reuveni M., Nissim-Levi A. and Oren-Shamir M. (2002). Increased anthocyanin accumulation in aster flowers at elevated temperatures due to Magnesium treatment. Physiol. Plant., 114: 559-565.

Dela G.S, Or E., Ovadia R., Nissim-Levi A., Weiss D. and Oren-Shamir M. (2003). Changes in anthocyanin concentration and composition in ‘Jaguar' rose flowers due to transient high-temperature conditions. Plant Sci., 164, 333-340.

Nissim-Levi A.S, Kagan S., Ovadia R. and Oren-Shamir M. (2003). Effects of temperature, UV-light and magnesium on anthocyanin pigmentation in Cocoplum leaves. J. of Hort. Sci. and Biotech., 78, 61-64.

Borovsky Y., Oren-Shamir M., Ovadia R., De Jong W. and Paran I. (2004). The A locus that controls anthocyanin accumulation in pepper encodes a MYB transcription factor homologous to Anthocyanin2 of Petunia Theor. Appl. Genet., 109, 23-29.

Ben-Yehudah G., Korchinsky R., Redel G., Ovadia R, Oren-Shamir M., and Cohen Y.(2005).
Colour accumulation patterns and anthocyanin biosynthetic pathway in 'Red Delicious' apple variants. J. of Hort. Sci. and Biotech., 80, 187-192.

Vaknin H.S, Bar-Akiva A., Ovadia R., Nissim-Levi A., Forer I., Weiss D. and Oren-Shamir M. (2005). Active anthocyanin degradation in Brunfelsia calycina (Yesterday-Toda-Tomorrow) flowers. Planta, 222, 19-26.

Nissim-Levi A.S, Ovadia R., Forer I. and Oren-Shamir M. (2007). Increased anthocyanin accumulation in ornamental plants due to magnesium treatment. J. of Hort. Sci. and Biotech. 82, 481-487.

Nissim-Levi A.S, Farkash L., Hamburger D., Ovadia R., Forer I., Kagan S. and Oren-Shamir M. (2008). Light-scattering shade net increases branching and flowering in ornamental pot plants.
J. of Hort. Sci. and Biotech. 83, 9-14.

Sapir M., Oren-Shamir M., Ovadia R., Reuveni M., Evenor D., Tadmor Y., Nahon S., Shlomo H., Chen L., Meir A. and Levin I. (2008). Molecular aspects of anthocyanin fruit tomato in 2.44; Category: Genetics and heredity; Rank 97/138

Ovadia R.S, Dori I., Nissim-Levi A., Shahak Y. and Oren-Shamir M. (2009). Coloured shade-nets influence stem length, time to flower, flower number and inflorescence diameter in four ornamental cut-flower crops. J. of Hort. Sci. and Biotech. 84, 161-166.

Lavi N., Tadmor Y., Meir A., Bechar A., Oren-Shamir M. Ovadia R., Reuveni M., Nahon S., Shlomo H., Chen, L. and Levin I. (2009). Characterization of the Intense Pigment tomato genotype emphasizing targeted fruit metabolites and chloroplast biogenesis. J. of Agri. and Food Chem. 57, 4818-4826

Lurie S.*, Oren-Shamir M.*, Ovadia R., Nissim-Levi A., Kaplunov T., Zutahy Y., Weksler H., and Lichter A. (2009). Abscisic acid improves color development of ‘Crimson Seedless' grapes both in the vineyard and on detached berries. J. of Hort. Sci. and Biotech. 84, 639-644.

Aharon Segev, Hana Badani, Yoram Kapulnik, Ilan Shomer, Michal Oren-Shamir and Shmuel Galili (2010). Determination of polyphenols, flavonoids and antioxidant capacity in colored chickpea (Cicer arietinum L.) J. of Food Science, 75, S115-119.

Liberman R.S, Shahar L., Nissim-Levi A., Evenor D., Reuveni M. and Oren-Shamir M. (2010).
Shoot regeneration from leaf explants of Brunfelsia calycina. Plant Cell Tissue and Organ Culture 100, 345-348.

Bar-Akiva A.S*, Ovadia R.S*, Rogachev I, Bar-Or C., Bar E., Freiman Z., Nissim-Levi A., Gollop N., Lewinshon E., Aharoni A., Weiss D., Koltai H and Oren-Shamir M. (2010). Metabolic networking in Brunfelsia calycina petals after flower opening. J. of Experimental Botany 61, 1393-1403.

Sinilal B.S, Ovadia R., Nissim-Levi A., Perl A., Weissberg M. and Oren-Shamir M. (2011).
Increased accumulation and decreased catabolism of anthocyanins in red grape cell suspension culture following magnesium treatment. Planta 234, 61-71.

Schreiber G., Reuveni M., Evenor D., Oren-Shamir M., Ovadia R., Sapir-Mir M, Bootbool-Man A, Nahon S, Shlomo H., Chen L, and Levin I (2011). Anthocyanin1 from Solanum chilense is more efficient in accumulating anthocyanin metabolites than its Solanum lycopersicum counterpart in association with the Anthocyaninin fruit phenotype of tomato.
Theor. Appl. Genet. 124, 295-307.

Ovadia R.S, Oren-Shamir M., Kapltinov T., Zutahy Y., Lichter A. and Lurie S. (2013).
Effects of plant growth regulators and high temperature on colour development in ‘Crimson Seedless' grapes. J. of Hort. Sci. and Biotech. 88, 387-392.

Nissim-Levi A.S, Kagan S, Ovadia R., Forrer I., Riov J. and Oren-Shamir M. (2013). Effects of temperature and auxin treatment on fruit set and pigmentation of Dodoneae 'Dana'. Sci. Hort. 160, 172-176.

Nissim-Levi A.S, Ovadia R., Kagan S. and Oren-Shamir M. (2014). Shading stock plants with photoselective nets affects the yield and rooting quality of their cuttings. J. of Hort. Sci. and Biotech. 89, 693-699.

Zipor G.S, Duarte P, Carqueijeiro I., Shahar L., Ovadia R., Teper-Bamnolker P., Eshel D., Levi Y., Doron-Faigenboim A., Sottomayor M. and Oren-Shamir M. (2014). In Planta Anthocyanin Degradation by a Vacuolar Class III Peroxidase in Brunfelsia Calycina Flowers. New Phytologist, in press.

Oliva M.S, Ovadia R., Perl A., Bar E., Lewinsohn E., Galili G. and Oren-Shamir M. (2014). Enhanced formation of aromatic amino acids increases fragrance without affecting flower longevity or pigmentation in Petunia x hybrid. Plant Biotech J., In press.


Updated on: 16/02/16 13:23
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