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Amit Gal-On, Ph.D.  (Researcher)
Plant ProtectionPlant Pathology and Weed Research
Research Interests / Job description  
Telephone Tel: 03-9683563 03-9683564 Lab 6312232154
Cellphone Cell: 050-6220563
Fax Fax: 03-9683918
Email Email: amitg@agri.gov.il  amitg@volcani.agri.gov.il
Office location Office location: Building of Plant Virology and Pathology. Room #101
Dept. of Plant Pathology
Volcani Center-ARO
P.O.Box 6
Bet-Dagan 50250
ISRAEL

 

Head, Dept. of Plant Pathology and Weed Research


 

Research Interests / Job description
The molecular basis of host and positive-strand RNA virus interaction; identification of plant genes associated with disease symptom development to plant virus infection.

Genetic engineering of attenuated ZYMV-AG as a plant virus vector, for gene expression and epitope presentation.

Development of resistance to RNA and DNA viruses, in cucurbits and tomato by transgenic approaches.

Transgene siRNA as a tool for control nematode and parasite weed.

 Publications

  •  Leibman, D., Wolf, D., Saharan, V., Zelcer, A., Arazi, T., Shiboleth, M. Y., Gaba, V., and Gal-On, A. (2011). A high level of transgenic viral small RNA is associated with broad potyvirus resistance in cucurbits. Mol. Plant-Microbe Interact. MPMI 24, 1220–1238.
  • Aly, R., Hamamouch, N., Abu-Nassar, J., Wolf, D., Joel, D. M., Eizenberg, H., Kaisler, E., Cramer, C., Gal-On, A., and Westwood, James H. (2011).Movement of protein and macromolecule between host plant and the parasitic weed Phelipanche aegyptiaca Pers.Plant Cell Reports. 30: 2233–2241.

  • Gaba V., Rosner A., Maslenin L., Leibman L., Singer S., Kukurt E., Shiboleth YM. and A. Gal-On. Breaking of siRNA-based virus resistance depends on homology of siRNA and challenge Virus. Eur J Plant Pathol (2010) 128:153-164
  • M. Lapidot, D. Guenoune-Gelbart, D. Leibman, V. Holdengreber, M. Davidovitz, Z. Machbash1, S. Klieman-Shoval, S. Cohen and A. Gal-On. (2010). Pelargonium zonate spot virus is transmitted vertically via seed and pollen in tomato. Phytopathology 100:798-804. 
  • Loebenstein, G., Rav David, D., Leibman, D., Gal-On, A., Vunsh, R., Czosnek, H. and Elad, Y. (2009). Tomato plants transformed with the inhibitor of virus replication (IVR) gene are partially resistant to Botrytis cinerea. Phytopathology 100: 225-229
  • Aly, R., Cholakh, H., Joel, D.M., Leibman, D., Steinitz, B., Zelcer, A., Naglis, A., Yarden, O. and Gal-On, A. (2009). Gene silencing of mannose 6-phosphate reductase in the parasitic weed Orobanche aegyptiaca through the production of homologous dsRNA sequences in the host plant.  Plant Biotechnol. J. 7: 487-498.
  • Gal-On, A., Naglis, A., Leibman, D., Ziadna, H., Kathiravan, K., Papayiannis, L., Holdengraber, V., Guenoune-Gelbert, D., Lapidot, M. and Aly, R. (2009). Broomrape can acquire viruses from its hosts. Phytopathology 99:1321-1329.
  • Shiboleth, Y. M., Haronsky, E., Leibman D., Arazi T, Whitham, S.  Wassenegger, M., Gaba V. and Gal-On, A. (2007). The conserved FRNK box in plant viral suppressor of gene silencing HC-Pro is required for small RNA binding and mediates symptom development. Journal Virology 81:13135-13148.
  • Harpaz-Saad, S., Azoulay, T., Arazi, T., Ben-Yaakov, E., Mett, A., Shiboleth, Y.M., Hörtensteiner, S., Gidoni, D., Gal-On, A., E. Goldschmidt, E. and Eyal, Y. (2007. Chlorophyllase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated. Plant Cell 19: 1007-1022.
  • Gal-On, A. (2006). Zucchini yellow mosaic virus: insect transmission and pathogenicity - the tails of two proteins. Molecular plant Pathology 8: 139-150.
  • Shoresh, M., Gal-On, A., Leibman, D. and Ilan Chet. (2006). Characterization of a MAPK gene from cucumber required for Trichoderma-conferred plant resistance. Plant Physiology 142: 1169-1179.
  • Gaba, V. and Gal-On, A. (2006). Inoculation of plants using bombardment. In: Current Protocols in Microbiology. Editors A. Simon, T. Kowalik, J. Quarles. Wiley Interscience. Volum 1 Unit 16B.4
  • Gal-On, A., Wolf, D., Antignus, Y., Patlis, L.,Ryu, K. H., Min, B. E., Pearlsman, M., Lachman, O., Gaba, G., Wang, Y., Yang, J. and Zelcer, A. (2005).Transgenic cucumbers harboring the 54-kDa putative gene of cucumber fruit mottle mosaic tobamovirus are highly resistant to viral infection and protect non-transgenic scions from soil infection. Transgenic research 14: 81-93.
  • Gal-On A. and Shiboleth, Y. M. (2005). Cross Protection. "Natural Resistance Mechanisms of Plants to Viruses" Ed. G. Loebenstein and J. P. Carr. Kluwer Academic Publishers, Netherlands
  • Aly, R., Mansour, F., Much, F. A., Edelstein, M., Meiri, E., Shiboleth, Y. M. and Gal-On, A. (2005). A novel approach to spider mite control based on expression of sarcotoxin IA peptide via virus-vector system in plants. Phytoparasitica 33: 177-186.
  • Kimalov, B., Gal-On, A., Stav, R., Belausov, E. and Arazi T. (2004) Maintenance of coat protein amino terminal net charge is essential for zucchini yellow mosaic virus systemic infectivity. J. Gen. Virology. 85: 3421-3430
  • Wang, Y., Tzfira, T., Gaba, V., Citovsky, V., Palukaitis, P. and Gal-On, A. (2004). Functional analysis of the cucumber mosaic virus 2b protein: pathogenicity and nuclear localisation. J. Gen. Virology. 85: 3135-3147
  • Gaba, V., Zelcer, A. and Gal-On, A. (2004). Cucurbit biotechnology - the importance of virus resistance. In Vitro Cellular & Developmental Biology. 40: 346-358.
  • Wang, Y., Lee, K. C., Gaba, V., Wong, S. M., Palukaitis, P. and Gal-On, A. (2004). Breakage of resistance to cucumber mosaic virus by co-infection with zucchini yellow mosaic virus: enhancement of CMV accumulation independent of symptom expression. Archives of virology, 249: 379 - 396.
  • Ullah, Z., Chai, B. L., Hammar, S; Raccah, B., Gal-On, A. and Grumet-R (2003). Effect of substitution of the amino termini of coat proteins of distinct potyvirus species on viral infectivity and host specificity. Physiological-and-Molecular-Plant-Pathology. 63: 129-139.
  • Desbiez, C., Gal-On, A. Girard, M. Wipf-Scheibel, C. and Lecoq H. (2003). Increase in zucchini yellow mosaic virus symptom severity in tolerant zucchini cultivars is related to a point mutation in P3 protein and is associated with a loss of relative fitness on susceptible plants. Phytopathology, 93:1478-1484
  • Ananthakrishnan G., Xia X., Elman C., Singer S., Paris H.S., Gal-On, A. and Gaba V. (2003). Shoot production in squash (Cucurbita pepo) by in vitro organogenesis. Plant Cell Reports, 21: 739-746.
  • Wang, Y., Gaba, V., Yang, J., Palukaitis, P. and Gal-On, A. (2002). Characterization of synergy between cucumber mosaic virus and potyviruses in cucurbit hosts. Phytopathology,92: 51-58
  • Arazi T, Lee Huang P, Huang PL, Zhang L, Moshe Shiboleth Y, Gal-On, A. and Lee-Huang S. (2002). Production of antiviral and antitumor proteins MAP30 and GAP31 in cucurbits using the plant virus vector ZYMV-AGII. Biochemical and Biophysical Research Communications 29:441-448
  • Shalitin, D., Wang, Y., Omid, A., Gal-On, A. and Wolf, S. (2002). Cucumber mosaic virus movement protein affects sugar metabolism and transport in tobacco and melon plant. Plant, Cell & Environment 25: 989-997.
  • Arazi, T., Shiboleth, Y. M., Wang. Y., Slutzky, G., Rubinstein, M., Barak, S., Yang, J. and Gal-On, A. (2001). Engineering zucchini yellow mosaic potyvirus as a non-pathogenic vector for expression of heterologous proteins in cucurbits. Journal Biotechnology 87: 67-82.
  • Antignus, Y., Wang, Y., Pearlsman, M., Lachman, O., Lavy, N. and Gal-On, A. (2001). Biological and molecular characterization of a new cucurbit-infecting tobamovirus. Phytopathology 91: 565-571.
  • Arazi, T., Shiboleth, Y. M. and Gal-On, A. (2001). A non-viral peptide can substitute for the entire N-terminus of zucchini yellow mosaic potyvirus coat protein and permit viral systemic spread. Journal of Virology 75: 6329-6336.
  • Shiboleth, Y. M., Arazi, T, Wang, Y. and Gal-On, A. (2001). A new approach for weed control in a cucurbit field employing an attenuated potyvirus-vector for herbicide resistance. Journal Biotechnology 92:37-46
  • Arazi, T., Shiboleth, Y. M. and Gal-On, A. (2001). A non-viral peptide can substitute for the entire N-terminus of zucchini yellow mosaic potyvirus coat protein and permit viral systemic spread. Journal of Virology 75: 6329-6336.
  • Wang, Y., Gaba, V., Wolf, D., Xia, X., Zelcer, A. and Gal-On A. (2000). Identification of a novel plant virus promoter using a potyvirus infectious clone. Virus Gene 20:11-17.
  • Gal-On, A., Canto, T. and Palukaitis, P. (2000). Characterization of genetically modified cucumber mosaic virus expressing histidine-tagged 1a and 2a proteins. Archives of Virology 145:37-50.
  • Gal-On, A and Raccah B. (2000). A point mutation in the FRNK motif of the potyvirus hc-pro gene alters symptom expression in cucurbits and exhibits protection against the severe homologous virus. Phytopathology 90:467-473.
  • Grumet, R., Ullah, Z., Gal-On, A. and Raccah, B. (2000). Cucurbit-potyvirus interactions influencing virus movement and accumulation. Acta Horticulturae 510: 327-334.
  • Fuchs, M., Gal-On, A., Raccah, B. and Gonsalves, D. (1999). Epidemiology of an aphid nontransmissible potyvirus in fields of non-transgenic and coat protein transgenic squash. Transgenic Research 8:429-439.
  • Gal-On, A., Meiri, E., Raccah, B. and Gaba, V. (1998). Recombination of engineered defective RNA species produces infective potyvirus in planta. Journal Virology 72: 5268-5270.
  • Gal-On, A., Wolf, D., Wang, Y., Faure, J. E., Pilowsky, and Zelcer, A. (1998). Transgenic resistance to CMV in tomato: blocking of long-distance movement of the virus in lines harboring a defective viral replicase gene. Phytopathology 88: 1101-1107.
  • Kadouri, D., Yan-hua Peng, Wang, Y., Singer, S., Huet, H., Raccah, B. and Gal-On, A. (1998). Affinity purification of HC-Pro of potyviruses with Ni2+-NTA resin. Journal of Virological Methods 76:19-29.
  • Gal-On, A., Meiri, E., Elman, C., Gray, D. J., and Gaba, V. (1997). Simple hand-held devices for the efficient infection of plants with viral-encoding constructs by particle bombardment. Journal of Virological Methods 64:103-110.
  • Kaplan, I., Gal-On, A. and Palukaitis, P. (1997). Characterization of cucumber mosaic virus. III. Localization of sequences in the movement protein controlling systemic infection in cucurbits. Virology 230:343-349.
  • Yang, G., Mawassi, M., Ashoulin, L., Gafny, R., Gaba, V., Gal-On, A. and Bar-Joseph, M. (1997). A cDNA clone from a defective RNA of citrus tristeza virus is infective in the presence of the helper virus. Journal General Virology 78:1765-1769.
  • Desbiez, D., Gal-On, A., Raccah, B. and Lecoq, H. (1997). Characterization of epitopes on zucchini yellow mosaic potyvirus coat protein permits studies on interactions between strains. Journal General Virology 78: 2073-2076.
  • Yan-hua Peng, Kadoury, D., Gal-On, A., Heut, H., Wang, Y. and Raccah, B. (1997). Mutation in the HC-Pro gene of the zucchini yellow mosaic potyvirus: Effects on aphid transmission and on binding to purified virions. Journal of General Virology 79: 897-904.
  • Gal-On, A., Kaplan, I. and Palukaitis, P. (1996). Characterization of cucumber mosaic virus. II. Identification of movement protein sequences that influence accumulation and systemic infection in tobacco. Virology 226: 354-361.
  • Gal-On, A., Kaplan, I. and Palukaitis, P. (1995). Differential effects of satellite RNA on the accumulation of cucumber mosaic virus RNAs and the encoded proteins in tobacco vs. zucchini squash with two strains of CMV helper virus.Virology 208:58-66.
  • Gal-On, A., Meiri, E., Huet, H., Hua, W. J., Raccah, B. and Gaba, V. (1995). Particle bombardment drastically increases the infectivity of cloned cDNA of zucchini yellow mosaic potyvirus. Journal General Virology 76:3223-3227.
  • Huet, H., Gal-On, A., Meiri, E., Huet, L. and Raccah, B. (1994). Mutations in the helper component (HC) gene of zucchini yellow mosaic virus affect its ability to mediate aphid transmissibility. Journal of General Virology 75: 1407-1414.
  • Carr, J. P., Gal-On, A., Palukaitis, P. and Zaitlin, M. (1994). Replicase-Mediated resistance to cucumber mosaic virus involves suppression of both virus replication in the inoculated leaves and long distance movement.Virology199: 439-447.
  • Gal-On, A., Kaplan, I., Roossinck, M. and Palukaitis, P. (1994). The kinetics of infection of zucchini squash by cucumber mosaic virus indicate a function for RNA 1 in virus movement. Virology 205:280-289.
  • Gal-On, A., Antignus, Y., Rosner, A. and Raccah, B. (1992). A zucchini yellow mosaic virus coat protein gene mutation restores aphid transmissibility but has no effect on multiplication. Journal of General Virology 73: 2183-2187.
  • Gal-On, A., Antignus, Y., Rosner, A. and Raccah, B. (1991). Infectious in vitro RNA transcripts derived from cloned cDNA of cucurbit potyvirus, zucchini yellow mosaic virus. Journal General Virology 72: 2639-2643
  • Gal-On, A., Antignus, Y., Rosner, A. and Raccah, B. (1990). Nucleotide sequence of the zucchini yellow mosaic virus capsid-coding gene and its expression in Escherichia coli. Gene 87: 273-277.
 

 

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