Shulamit Manulis-Sasson, Ph.D.

Name:	Shulamit Manulis-Sasson, Ph.D. (Retiree)
Units:	Plant Protection, Plant Pathology and Weed Research
Research Interests / Job description

Tel:	03-9683685 03-9683687
Cell:	050-6220685
Fax:	03-9683686
Email:	shulam@volcani.agri.gov.il
Office location:	Bet Dagan
Address:	Dept. of Plant Pathology ARO, The Volcani Center Derech Hamacabim 68 Bet Dagan 50250 ISRAEL
Senior Researcher

Research Interests / Job description

Studies on molecular basis of bacterial-host interactions, cloning and characterization of pathogenicity genes, development and implementation of molecular techniques for detection of major and economically important bacterial diseases: Pantoea agglomerans pv. gypsophilae, Xanthomonas campestris pv. pelargonii, Clavibacter michiganensis subsp. michiganensis, Erwinia amylovora, Agrobacterium tumefaciens.


Selected Publications Valinsky, L., Nisan, I., Tu, X., Nisan, G., Rosenshine, I., Hanski, E., Barash, I. and Manulis, S. (2002). A host-specific virulence protein of Erwinia herbicola pv. gypsophilae is translocated into human epithelial cells by the type III secretion system of enteropathogenic Escherichia coli. Molecular Plant Pathology 3:97-101. Manulis, S., Chalupowicz, L., Dror, O. and Kleitman, F. (2002). Molecular diagnostic procedures for production of pathogen-free propagation material. Pest Management Science 58:1126-1131. Shtienberg, D., Shwartz, H., Oppenheim, D., Zilberstaine, M., Herzog, Z., Manulis, S. and Kritzman, G. (2003). Evaluation of local and imported fire blight warning systems in Israel. Phytopathology 93:356-363. Nizan-Koren, R., Manulis, S., Mor, H., Iraki, N. M. and Barash, I. (2003). The regulatory cascade that activates the hrp regulon in Erwinia herbicola pv. gypsophilae. Molecular Plant Microbe Interactions 16:249-260. Manulis, S., Kleitman, F., Shtienberg, D., Shwartz, H., Oppenheim, D., Zilberstaine, M., and Shabi, E. (2003). Changes in the sensitivity of Erwinia amylovora populations to streptomycin and oxolinic acid in Israel. Plant Disease 87:650-654. Kritzman, G., Shwartz, H., Marcus, R., Manulis, S., Kleitman, F., Oppenheim, D., Zilberstaine, M. and Shtienberg, D. (2003). Testing a rapid diagnostic medium for Erwinia amylovora and development of a procedure for sampling blossoms in pear orchards. Phytopathology 93:931-940. Kleitman, F., Manulis, S., Kritzman, G., Oppenheim, D., Zilberstaine, M. and Shtienberg, D. (2004). Use of a diagnostic medium for in situ determination of the response of Erwinia amylovora strains to bactericides. Phytoparasitica 32:127-131. Ezra, D., Barash, I., Weinthal, D.M., Gaba, V. and Manulis, S. (2004). pthG from Pantoea agglomerans pv. gypsophilae encodes an avirulence effector that determines incompatibility in multiple beet species. Molecular Plant Pathology 5:105-114. Kleitman, F., Shtienberg, D., Blachinsky, D., Oppenheim, D., Zilberstaine, M., Dror, O. and Manulis, S. (2005). Erwinia amylovora populations resistant to oxolinic acid in Israel: prevalence, persistence and fitness. Plant Pathology 54:108-115. Hadas, R., Kritzman, G., Kleitman, F., Gefen, T. and Manulis, S. (2005). Comparison of extraction procedures and determination of the detection threshold for Clavibacter michiganensis subsp. michiganensis in tomato seeds. Plant Pathology 54:643-649. Nissan, G., Manulis, S., Weinthal, D.M., Sessa, G. and Barash, I. (2005).Analysis of promoters recognized by HrpL, an alternative sigma factor protein from Pantoea agglomerans pv. gypsophilae. MPMI 18:634-643. Chalupowicz, L., Barash, I., Schwartz, M., Aloni, R. and Manulis, S. (2006). Comparative anatomy of gall development on Gypsophila paniculata induced by bacteria with different mechanisms of pathogenicity. Planta 224:429-437. Blachinsky, D., Shtienberg, D., Zamski, E., Weinthal, D. and Manulis, S. (2006). Effects of pear tree physiology on fire blight progression in perennial branches and on expression of pathogenicity genes of Erwinia amylovora. European Journal of Plant Pathology 116:315-324. Nissan, G., Manulis-Sasson, S., Weinthal, D., Mor, H., Sessa, G. and Barash I. (2006). The type III effectors HsvG and HsvB of gall forming Pantoea agglomerans determine host specificity and function as transcriptional activators. Molecular Microbiology 61:1118-1131. Weinthal, D.M., Barash, I., Panijel, M., Valinsky, L., Gaba, V. and Manulis-Sasson, S. (2007). Distribution and replication of the pathogenicity plasmid pPATH in diverse populations of the gall-forming Pantoea agglomerans. Applied and Environmental Microbiology 73:7552-7561. Kleitman, F., Barash, I., Burger, A., Iraki, N., Falah, F., Sessa, G., Weinthal, D., Chalupowicz, L., Gartemann, K.-H., Eichenlaub, R. and Manulis-Sasson, S. (2008). Characterization of Clavibacter michiganensis subsp. michiganensis population in Israel. European journal of Plant Pathology 121:463-475. Balaji, V., Mayrose, M., Sherf, O., Jacob-Hirsh, J., Eichenlaub, R., Iraki, N., Manulis-Sasson, S., Rechavi, G., Barash, I. and Sessa, G. (2008).Tomato transcriptional changes in response to Clavibacter michiganensis subsp. michiganensis reveal a role for ethylene in disease development. Plant Physiology 146:1797-1809. Chalupowicz, L., Manulis-Sasson, S., Itkin, M., Sacher, A., Sessa, G. and Barash I. (2008). Quorum sensing system affects gall development incited by Pantoea agglomerans pv. gypsophilae. Molecular Plant-Microbe Interactions 21:1094-1105. Chalupowicz, L., Barash, I., Panijel, M., Sessa, G. and Manulis-Sasson, S. (2009). Regulatory interactions between quorum-sensing, auxin, cytokinin and the hrp regulon in relation to gall formation and epiphytic fitness of Pantoea agglomerans pv. gypsophilae. Molecular Plant-Microbe Interactions 22:849-856. Chalupowicz, L., Cohen-Kandli, M., Dror, O., Eichenlaub, R., Gartemann, K.-H., Sessa, G., Barash, I. and Manulis-Sasson S. (2010). Sequential expression of bacterial virulence and plant defense genes during infection of tomato with Clavibacter michiganensis subsp. michiganensis. Phytopathology 100:252-261. Weinthal, D.M., Yablonski, S., Singer, S., Barash, I., Manulis-Sasson, S. and Gaba, V. (2010). The type III effector PthG of Pantoea agglomerans pv. gypsophilae modifies host plant responses to auxin, cytokinin and light. European Journal of Plant Pathology 128:289-302. Weinthal, D.M., Barash, I., Tzfira, Z., Gaba, V., Teper, D., Sessa, G. and Manulis-Sasson, S. (2011). Characterization of nuclear localization signals in the type III effectors HsvG and HsvB of the gall-forming bacterium Pantoea agglomerans. Microbiology 157:1500-1508. Savidor, A., Teper, D., Gartemann, K.-H., Eichenlaub, R., Chalupowicz, L., Manulis-Sasson S., Barash, I., Tews, H., Mayer, K., Giannone, R.J., Hettich, R.L. and Sessa, G. (2012). The Clavibacter michiganensis subsp. michiganensis-tomato interactome reveals the perception of pathogen by the host and suggests mechanisms of infection. J. Proteome Research 11:736-750. Chalupowicz, L., Zellermann, E.-M., Fluegel, M., Dror, O., Eichenlaub, R., Gartemann, K.-H., Savidor, A., Sessa, G., Iraki, N., Barash, I. and Manulis-Sasson,S. (2012). Colonization and movement of GFP-labeled Clavibacter michiganensis subsp. michiganensis during tomato infection. Phytopathology 102:23-31. Nissan, G., Manulis-Sasson, S., Chalupowicz, L., Teper, D., Yeheskel, A., Pasmanik-Chor, M., Sessa, G. and Barash, I. (2012). The type III effector HsvG of the gall-forming Pantoea agglomerans mediates expression of the host gene HSVGT. Molecular Plant-Microbe Interactions 25:231-240. Sharabani, G., Manulis-Sasson, S., Borenstein, M., Shulhani, R., Lofthouse, M., Chalupowicz, L. and Shtienberg, D. (2013).The significance of guttation in the secondary spread of Clavibacter michiganensis subsp. michiganensis in tomato greenhouses. Plant Pathology 62:578-586. Chalupowicz , L., Weinthal, D., Gaba, V., Sessa, G., Barash, I. and Manulis-Sasson, S. (2013). Polar auxin transport is essential for gall formation by Pantoea agglomerans on gypsophila. Molecular Plant Pathology 14:185-190. Sharabani, G., Shtienberg, D., Borenstein, M., Shulhani, R., Lofthouse, M., Sofer, M., Chalupowicz, L., Barel, V. and Manulis-Sasson, S. (2013). Effects of plant age on disease development and virulence of Clavibacter michiganensis subsp. michiganensis on tomato. Plant Pathology (in press). Tsror (Lahkim), L., Ben-Daniel, B., Chalupowicz, L., van der Wolf, J., Lebiush, S., Erlich, O., Dror, O., Barel, V., Nijhuis, E. and Manulis-Sasson, S. (2013). Characterization of Dickeya strains isolated from potato grown under hot-climate conditions. Plant Pathology (in press). Panijel, M., Chalupowicz, L., Sessa, G., Manulis-Sasson, S. and Barash, I. (2013).Global regulatory networks control the Hrp regulon of the gall-forming bacterium Pantoea agglomerans pv. gypsophilae. Molecular Plant-Microbe Interactions (in press). Chapters in Books and Reviews Barash, I., Dori, S., Mor, H., and Manulis, S. (1993). Role of iron in fungal phytopathologies. In: Iron chelation in plants and soil microorganisms. Barton, L.L. and Hemming, B.C. eds. Academic Press, NY. Manulis, S. (1998). Detection of plant pathogens by serological and molecular methods. In: Plant Diseases in Israel. Rotem, J., Palti, J., and Ben-Yephet, Y. eds. (in Hebrew). Ben-Yephet, Y., Palti, J. and Manulis, S. (1998). Fusarium oxysporum : epidemiology and control. . In: Plant Diseases in Israel. Rotem, J., Palti, J., and Ben-Yephet, Y. eds. (in Hebrew). Manulis, S. and Barash, I. (2003). The molecular basis for transformation of an epiphyte into a gall-forming pathogen as exemplified by Erwinia herbicola pv. gypsophilae, (Stacey, G. and Keen, N.T. eds.). Plant-Microbe Interactions 6:19-52. Manulis, S. and Barash, I. (2003). Pantoea agglomerans pvs. gypsophilae and betae, recently-evolved pathogens? Molecular Plant Pathology 4:307-314. Barash, I. and Manulis S. (2005). Hrp-dependent biotrophic mechanism of virulence - how it has evolved in tumorigenic bacteria? Phytoparasitica 33:317-324. Barash, I. and Manulis-Sasson, S. (2007). Virulence mechanisms and host specificity of gall-forming Pantoea agglomerans. Trends in Microbiology 15:538-545. Barash, I. and Manulis-Sasson, S. (2009). Recent evolution of bacterial pathogens: the gall-forming Pantoea agglomerans case. Annual Review of Phytopathology 47:133-152.

Updated on: 04/04/19 09:44