Yedidya Gafni, Prof.

Name:	Yedidya Gafni, Prof. (Retiree)
Units:	Plant Sciences, Vegetable and Field Crops
Research Interests / Job description

Tel:	03-9683471
Cell:	050-6220471
Fax:	03-9683471
Email:	ygafni2@gmail.com ygafni@volcani.agri.gov.il
Office location:	Plant Sciences Institute Bld. Room 105
Address:
Department of Genetics and Vegetable Research

Research Interests / Job description

The research in my laboratory is aimed at understanding the molecular mechanisms involved in the interactions between the Tomato yellow leaf curl virus and the host plant cell. Recently we discovered the suppression of gene silencing activity of the V2 protein of the virus. The understanding of the mechanism(s) by which V2 interferes with the silencing machinery of the host plant cell is now under investigation.


Link to Wikipedia(Hebrew only): https://he.wikipedia.org/wiki/%D7%99%D7%93%D7%99%D7%93%D7%99%D7%94_%D7%92%D7%A4%D7%A0%D7%99 Recent publications: 1. Krichevsky, A., Kozlovsky, S.V., Gafni, Y. and Citovsky, V.(2006) Nuclear import of plant viral proteins and genomes. Mol. Plant. Pathology 7: 131-146 2. Pravin Kumar, P., Usha, R. Zrachya, A., Levy, Y., Spanov, H. and Gafni, Y.(2006) Protein-Protein interactions and nuclear trafficking of Coat protein and bC1 protein associated with Bhendi yellow vein mosaic disease. Virus Research 122: 127-136. 3. Citovsky, V., Lee, L.Y., Vyas, S., Kozlovsky, S.V., Glick, E., Vainstein, A., Gafni, Y., Gelvin, S.B., & Tzfira, T. (2006) Identification of subcellular localization of interacting proteins by bimolecular fluorescence complementation in planta. J. Mol. Biol. 362: 1120-1131. 4. Zrachya, A., Pravin Kumar, P., Usha, R., Levy, Y., Loyter, A., Arazi, T., Lapidot, M. and Gafni, Y. (2007) Production of siRNA targeted against TYLCV coat protein transcripts leads to silencing of its expression and resistance to the virus. Transgenic Research 16: 385-398. 5. Zrachya, A, Glick, E., Levy, Y., Arazi, T., Citovsky, V. and Gafni, Y. (2007)Suppressor of RNA silencing encoded by Tomato yellow leaf curl virus-Israel. Virology 358: 159-165. 6. Zaltsman, A., Bu-Young, Y., Krichevsky, A., Gafni, Y. and Citovsky, V. (2007)Yeast-Plant Coupled Vector System for Identification of Nuclear Proteins Plant Physiol.145: 1264-1271. 7. Glick, E, Zrachya, A., Levy, Y., Mett, A., Gidoni, D., Belausov, E., Citovsky, V. and Gafni, Y. (2008) Interaction with host SGS3 is required for suppression of RNA silencing by Tomato yellow leaf curl virus V2 protein. Proc. Nat. Acad. Sci. USA 105: 157-161. 8. Citovsky, V., Gafni, Y. and Tzfira, T. (2008) Localizing protein-protein interactions by bimolecular fluorescence complementation in planta. Methods 45: 196-206. 9. Glick, E., Levy, Y. and Gafni, Y. (2009) The viral etiology of tomato yellow leaf curl disease - a review. Plant Protect. Sci., 45: 81 - 97. 10. Citovsky, V., Zaltsman, A., Kozlovsky, SV., Gafni, Y. and Krichevsky, A. (2009) Proteasomal degradation in plant-pathogen interactions. Semin. Cell Dev. Biol. 20: 1048-1054. 11. Solmwsky, LJ., Zrachya, A., Denisova, G., Gafni, Y. and Gershoni, J. (2010) Preparation and epitope characterization of monoclonal antibodies suitable for detection of Tomato yellow leaf curl virus. Phytoparasitica 38: 201-208. 12. Yaakov, N., Levy, Y.,Belausov, E., Gaba, V., Lapidot, M. and Gafni, Y. (2011) Effect of a single amino acid substitution in the NLS domain of Tomato yellow leaf curl virus-Israel (TYLCV-IL) capsid protein (CP) on its activity and on the virus life cycle. Virus Res. 158: 8-11. 13. Rand, K., Kobrinsky-Aaronowitz, I., Levy, Y., Shaul, O., Aloni, R. and Gafni, Y. (2011) Induction of karyopherin α1 expression by indole-3-acetic acid in auxin-treated or overproducing tobacco plants. Plant Signal Behav. 6: 815-820. 14. Chandran, S.A., Levy, Y., Mett, A., Belausov, E., Ramakrishnan, U. and Gafni, Y. (2012) Mapping of functional region conferring nuclear localization and karyopherin alpha-binding activity of the C2 protein of bhendi yellow vein mosaic virus. J. Gen. Virol. 93:1367-1374. 15. Bar-Ziv, A., Levy, Y., Hak, H., Mett, A., Belausov, E., Citovsky, V. and Gafni, Y. (2012) The Tomato yellow leaf curl virus (TYLCV) V2 protein interacts with the host papain-like cysteine protease CYP1. Plant Signal Behav. 7: 983-989. 16. Oteng-Frimpong, R., Levy, Y., Torkpo, S.K., Danquah, E.Y., Offei, S.K. and Gafni, Y. (2012) Complete genome sequencing of two causative viruses of cassava mosaic disease in Ghana. Acta Virol. 56: 305-314. 17. Girshengorn, M., Zaltsman, A., Singh, L., Levy, Y. and Gafni, Y. (2012) siRNA targeted against TYLCV V2 protein transcripts leads to inhibition of its activity as a suppressor of RNA silencing. Current Topics in Virol. 10: 31-38. 18. Zaltsman, A., Lacroix, B., Gafni, Y. and Citovsky, V. (2013) Disassembly of synthetic Agrobacterium T-DNA-protein complexes via the host SCF(VBF) ubiquitin-ligase complex pathway. Proc. Nat. Acad. Sci. USA 110: 169-174. 19. Bar-Ziv, A., Levy, Y., Citovsky, V. and Gafni, Y. (2015) The Tomato yellow leaff curl virus(TYLCV) V2 protein inhibits enzymatic activity of the host papain-like cysteine protease CYP1. Biochem. Biophys. Res. Comm. 460: 525-529. 20. Hak, H. Levy, Y. Chandran, S.A., Belausov, E., Loyter, A., Lapidot, M. and Gafni, Y. (2015) TYLCV-Is movement in planta does not require V2 protein. Virology 477: 56-60.

Updated on: 21/06/16 11:35