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Reactive oxygen species (ROS) in senescnece
Senescence process has implication to the global food crises, since it affects yield and shelflife maintenance of fruit and leaves after harvest. Senescence is a regulated process in which ROS production and oxidative stress play a major role. Our goal is to characterize the ROS response and to elucidate the role of ROS in senescence.
Increase in ROS in different stresses can serve as a signal for activation of multitudes of genes which are related to protection or to cell death. Our working hypothesis is that oxidative stress in different cellular sites (plastids, cytoplasm, mitochondria, and peroxisomes) will activate a different set of genes. Our goal is to identify the  site(s) in which oxidative stress occurs at early stages of senescence and the components involved in ROS signal transduction. We are using Arabidopsis transgenic plants expressing a redox sensitive probe (roGFP) in different subcellular organelles. We found that this protein is oxidized in the mitochondria at early stages of senescence. This finding can lead to the discovery of components involved in ROS signal transduction during senescence.  (The picture display localization of roGFP to mitochondria.)
21/01/13 | Monday
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